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Antibodies to PKD, p-PKD (916), p-PKD (744/748), and PKD substrates were from Cell Signaling Technology (Herts, UK).
Annexin V-FITC, rTRAIL (super killer TRAIL), caspase-3 and -9 substrates were from Enzo Life Sciences AG Lausenn, Switzerland).
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BCA Bradford protein assay kit and super signal West pico chemiluminescent substrate were from Pierce (Rockford, IL, USA).
Horseradish peroxidase conjugated secondary antibodies to mouse or rabbit IgG and chemiluminescent substrate (SuperSignal West Dura Extended Duration Substrate) were from Pierce (Rockford, IL).
The secondary antibody multi-link-biotin, the streptavidin-HRP-label and the AEC-substrate were from Dako and were used according to the manufacturer's instruction.
Most residues in contact with the S1 substrate were from TMs1, 3, 6 and 8 and remained the same in both trajectories (Figure 1B, Table 1).
Protease inhibitors and chemiluminescence substrate were from Roche Diagnostics.
The anti-mouse peroxidase-conjugated antibody and the SuperSignal chemiluminescent substrate were from Pierce (Rockford, IL).
Super Signal® West Pico and chemiluninescent substrate were from Pierce Biotechnology (Rockford, IL, USA).
Peroxidase labeled rabbit anti-human immunoglobulin and ABTS peroxidase substrate were from KPL (Gaithersburg, MD, USA).
BCA™ Protein Assay Kit and Super Signal West Pico Chemiluminescent substrate were from Pierce Biotechnology, Inc. (Rockford, IL, USA).
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