Sentence examples for substrates were fixed from inspiring English sources
The substrates were fixed at 1, 3 and 5 cm distance from this shadowing block along four mutually normal radii of the substrate holder disc.
At defined time points, the HUVECs cultured on experimental substrates were fixed with 4%% paraformaldehyde in PBS for 20 min at room temperature.
The circular substrates were fixed with double-sided tape to a rectangular prism that was attached to the axis of a stepper motor configured to move in steps of 9°.
Substrates were fixed with 2% glutaraldehyde in 0.2 M sodium cacodylate buffer, pH 7.4 for 24 hr at 4°C, and dehydrated by exposure to a graded ethanol-water series, and stored in 100% ethanol in plates.
After 24 hours of incubation, the cells on substrates were fixed in 3.7% (v/v) formaldehyde for 30 min.
To examine focal adhesion formation and cytoskeletal organisation, osteocytes cultured on normal and OA ECM substrates were fixed in 4% paraformaldehyde (pH 7.4 in PBS) for 10 minutes, and then permeabilised with 0.2% Triton X-100 for 10 minutes.
It is noted that in the validation simulation, the hydroxyl groups on the surface of silicon dioxide substrates are fixed as other representative works done.
During spraying, the nozzle and substrate were fixed, and the thickness of EML was controlled by varying the deposition time.
The bottom outer layers of atoms in the substrate were fixed in space, and the layers neighboring them were kept at a constant temperature of 293 K to imitate heat dissipation in a real nanoindentation condition.
The adherent cells on the substrate were fixed for 1 h in 4% paraformaldehyde at room temperature, followed by two washes in phosphate buffered saline (PBS) and permeabilization with 0.1% Triton X-100(Sigma-Aldrich) in PBS for 15 min at 4 °C.
Most results reported have been obtained from the harmonic quantum transition state theory method (also known as instanton) on an analytic potential specifically fitted to a DFT potential energy surface in which the atoms in the substrate were fixed at the positions they adopt prior to H2 dissociation.
