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Exact(6)
After this time, the substrates were extracted, washed with fresh toluene/EtOH and acetone, and dried under vacuum.
Next, the specific growth temperature was maintained for reaction times varying from 30 to 120 min. Finally, the furnace was cooled down to room temperature, and the substrates were extracted from the tube furnace.
Lipids and substrates were extracted by adding 1 volume chloroform/methanol (2 1, v/v).
To investigate the correlations between CIs and the kinase families of these motifs, data describing the relationships between kinases and substrates were extracted from PhosphoSitePlus [ 13, 14].
Polymers were disintegrated; the template and non-reacted substrates were extracted with a methanol and acetic acid mixture (96:4% v/v) in a Soxhlet apparatus over 24 h, because ASE did not fully remove the template.
To investigate the stability of the grafted pSBMA brushes, the Ti-pSBMA substrates were subjected to bath-sonication in TFE for 30 min (note that all substrates were extracted in TFE, a good solvent for free pSBMA, for 24 h to remove physically absorbed free polymers prior to sonication).
Similar(54)
All substrates are extracted from the ER by the associated Cdc48 complex before degradation by the 26S proteasome.
Ubiquitinated ERAD substrates are extracted from the ER lumen (ERAD L substrates) or from the ER membrane (M/C substrates) by a trimeric complex of the homohexameric proteins cdc48 (p97 or valosin-containing protein in mammals), Ufd1 and Npl4 (each harboring an ubiquitin-binding domain) [ 54].
Samples (25 μL) were withdrawn after reaction for a certain time, and then the product and residual substrate were extracted with ethyl acetate (2 × 25 μL, 5 mM n-dodecane as internal standard) before GC analysis.
After 24 h of incubation, product and remaining substrate were extracted thrice by hexane addition in a 1 1 ratio.
After 24 h of incubation, product and remaining substrate were extracted twice by ethyl acetate addition in a 1 1 ratio.
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