Sentence examples for substrates were estimated from inspiring English sources
Actually, using this time course of enzymatic reactions, kinetic constants of rat liver 2,6-sialyltransferase against glycosyl donor substrates were estimated to be Km = 4.85 μM and Vmax = 0.119 μmol/min, respectively.
Steady-state kinetic constants for oxidation of representative substrates were estimated for the E. coli-produced native (wild-type) MnP6 from C. subvermispora, its S168W and S168W-environment variants, P. chrysosporium LiP-H8, and P. ostreatus VP1.
Since the correct wavelength, which can be solved from Fig. 7, is 609.3 nm for 0% oxygen level, the respective errors of the oxygen level for the substrates were estimated to be 0.0%, 4.5%, and −2.2% from Eq. (5).
Finally, the binding energies and binding entropies of the substrates were estimated according to the method developed by Tirado-Rives et al. (Tirado-Rives and Jorgensen 2006) using the same reference geometry as for the enzyme without substrate (with active site residues minimized in 12 Å radius from the Tyr OH group).
Static friction force between NWs (including CNTs) and substrates was estimated from the highly deformed shapes of NWs [11].
The crystallinity degree of thin polyamide films (PA66, 610 and 612) spin coated on four types of substrates are estimated.
The concentration of DNA substrates was estimated by relating the specific activity of the labelled oligonucleotide to the activity of the purified substrate, and is expressed in molar concentrations of DNA substrate.
Therefore, the elasticity of those substrates was estimated by extrapolating existing calibrations.
The affinity of each GS isoenzyme for the substrates was estimated using the synthetase reaction and 10 μg (GS1a; GS2andnd GS2b) or 20 μg (GS1b) of purified protein per assay.
The data were analyzed with a detailed model of carbon transitions in the TCA cycle and two primary flux parameters of interest (reflecting total aerobic metabolism and uptake of the labeled substrate) were estimated.
The interaction energy between this carbon atom and the substrate is estimated by (2).
