Sentence examples for substrates were dissolved from inspiring English sources

All substrates were dissolved in 250 mM potassium phosphate buffer (pH 6.5).

All substrates were dissolved in 50 75% dimethyl sulfoxide to 20 mM.

Enzymes and substrates were dissolved in 50 mM Tris-HCl, pH 8.0 containing 0.01% Triton X-100 (v/v), and reactions were initiated by the addition of enzyme.

The substrates were dissolved in DMSO or dioxane.

Peptide substrates were dissolved in 50 mM Tris-HCl, pH 7.6.

The carboxylate ester substrates were dissolved in DMSO (100 mM) and then diluted into the reaction mixture.

To determine the optimal pH for cellulase and xylanase, substrate were dissolved in 0.05 M phosphate buffer (pH 5.2 7.7) and 0.05 M glycine-NaOH buffer (pH 8.6 9.8).

Then the MTT was aspirated, and the formazan crystals resulting from mitochondrial enzymatic activity on the MTT substrate were dissolved with 150 μL of DMSO; absorbance at 490 nm was recorded by the multiwell-plate reader at 490 nm.

Catalyst and substrate were dissolved in (a) 99.8% methanol- d4 (Carl Roth, Germany), (b) 100% ethanol (Sigma-Aldrich), or (c) 100% ethanol followed by dilution with water in the ratio 1 9.

Maltotriose as a substrate was dissolved in D2O containing 50 mM 2-morpholinoethanesulfonic acid (MES -NaOH (pH 6.0).

For the preparative scale, approximately 100 mg of substrate was dissolved in phosphate buffer (50 mM potassium phosphate, 2 mM DTT, 1 mM EDTA, pH 7.5) in a round-bottomed flask.