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After purification, the catalytic activities towards different substrates were determined.
The adsorption isotherms of cellulosic substrates were determined as follows.
Characteristics of the substrates were determined beforehand as demonstrated by Figure13 and Figure14.
Kinetic constants for synthetic and natural substrates were determined using Hanes plots (Table 1).
Elemental compositions of substrates were determined with an elemental analyzer (Vario EL cube, Germany).
The site densities of immobilized P-selectin on substrates were determined by the 125I radioiodination method (Ling et al., 2013).
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Composition of raw untreated substrates and washed pretreated substrates was determined according to the laboratory analytical procedure "Determination of structural carbohydrates and lignin in biomass" from National Renewable Energy Laboratory NRELL) [ 24].
In addition, the enzyme activity for the substrates was determined under varying H2O2 concentrations (0 1250 µM).
The thickness of native SiO2 layer on Si substrates was determined by ellipsometry as 1.5 nm.
Degree of deacetylation of the substrates was determined using FT-IR and elemental analysis.
From the signs of the Cotton effects, the absolute configuration of the substrates is determined.
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