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The surface roughness of the substrates was obtained by atomic force microscopy and the morphology of the laminate surfaces was analysed by scanning electron microscopy.
For example, a fast (a few hours instead of 14 days with SBF) biomimetic deposition of CDHA coatings on Ti6Al4V substrates was obtained using a slightly supersaturated Ca/P solution with an ionic composition simpler than that of SBF.
A semi-quantitative estimate of C. gattii attachment to the natural or artificial substrates was obtained similarly to our quantification of mating in SEM images described previously [10].
The total area spreading by RB or POS (plasma membrane bound + ingested) was obtained from group 1, and the area spreading by plasma membrane-bound RB or POS was obtained from group 2. The area spreading by ingested substrates was obtained by subtracting the area of plasma membrane-bound particles from total area of particles bound and ingested by Sertoli cells.
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The ZnO nanotubes by aqueous chemical growth (ACG) technique on p-GaN substrates were obtained.
The height differences between graphene areas and SiO2 substrates are obtained in the same way.
Uniform particle monolayers on Si substrates were obtained after appropriate adjustment of the particle concentration.
All chemicals for media and substrates were obtained from Sigma-Aldrich Canada Co. (Oakville, ON, Canada).
From fluorescence microscopy, limited information on cell morphology to qualify the cell development on these silicon substrates is obtained.
The synthetized substrates are obtained with a facile and reproducible method from poly-N-isopropylacrylamide and superparamagnetic iron oxide nanoparticles.
Moreover, the corresponding Mo coatings deposited onto different substrates were obtained.
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