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In this work, we describe the results of screening of a library of fluorogenic substrates to identify non-natural amino acids that are optimally recognized by BLMH.
The same process was applied to the other substrates to identify specific kinase-substrate interactions for 6 of the 7 protein kinases.
The analytical profile index (API) test was used with 50 different sugars as substrates to identify growing and non-growing L. plantarum strains on these sugars [ 8].
After covalently attaching streptavidin onto the PLGA nanofibers, conjugation of biotinylated antibodies confers capture specificity to PLGA NanoVelcro substrates to identify CTCs from whole blood samples.
Mitochondrial ROS Production in wild-type strains mtROS production was measured in 10 day old flies using two different substrates to identify which ETC complex or complexes (if any) are implicated in variation of mtROS production.
These adducts were shown to be useful substrates for polyP-degrading enzymes, allowing us to monitor enzyme activity spectrophotometrically in real time; furthermore, we used these substrates to identify a new function for the clinically significant enzyme, Nudt2.
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Activity assays were performed using 2,6-DMP as substrate to identify the pH and temperature properties.
For the optical study of a single object, the products were dispersed onto a marked substrate to identify and locate the positions of the individual microcavities.
Since we had the ability to modify the nucleotide sequence at will, we designed and tested several variations of the THF-containing red substrate to identify the optimal arrangement for the screening assay.
Four hours after the application of the particles to the medium of podocytes, the cells were incubated with X-gal substrate to identify podocytes that have taken up enzymatically active β-galactosidase.
Thus, this yeast system allows us to develop a high-throughput screening method, without using radioactive substrate, to identify aromatase inhibitors as well as new ligands (nonaromatizable androgen mimics) for the androgen receptors.
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