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To test Eng2 activity with different oligoglucan substrates, the amount of reducing sugars formed was estimated as described previously (Fontaine et al., 1997).
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On the YDC substrates, the amounts of carbon deposited on the Ni surface were reduced due to their oxide ion conductivity and proton solubility.
At a given concentration of substrate, the amount of products rapidly reached a plateau with the increasing concentrations of enzyme.
In relation to elevated trace element concentrations in the Si-waste substrate, the amount of metals accumulated in the tissues of all tested taxa was relatively low.
For the substrate, the amount of plated nickel was reduced to less than half compared with the conventional foam-type substrate, and the manufacturing process was simplified, which lowers the manufacturing cost of the substrate.
When NaCl is added to the substrate, the amount eaten differs depending on NaCl concentration.
Indeed, RFC's affinity for PCNA decreases ∼3 orders of magnitude in the presence of ADP alone (Zhang et al., 1999; Shiomi et al., 2000) and if Neutravidin is omitted from the Cy3-P/T DNA substrate, allowing PCNA to slide off the primer end of the Cy3-P/T DNA substrate, the amount of Cy5-PCNA unloaded by RFC decreases by 2.3 ± 0.27-fold.
The variation in different reports could be due to the composition of substrates and the amount of fat existing in food.
The pH value would be a critical parameter influencing the distribution and density of deposited AuNPs on substrates since the amount of negative charge would affect agglomeration and precipitation in the following steps.
The availability of the substrates affects the amount of isoamyl acetate produced.
After removal of unbound substrate, the amounts of protein and DNA were determined by fluorescence intensity measurements with a Tecan Infinite M1000 plate reader using calibration curves from purified GFP or DNA coupled ATTO fluorophores, respectively.
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