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We used 6H-SiC single crystal substrates, supplied by the Crystal Bank at Pusan National University.
After taking into account the labeling of substrates supplied by the metabolically active cob, the fluxes through central metabolism were quantified by computer-aided modeling.
Growth inhibition of biomass adapted to the degradation of toluene and xylenes in mixture in a batch reactor with substrates supplied by pulses.
Then, 7.0 wt.% PMMA and 2.8 wt.% FR solutions were mixed and spin-coated onto freshly cleaned silicon wafers (crystallographic orientation (100), resistance 0.002 Ω cm, refractive index n = 3.50) or glass substrates (supplied by Glassbel Ltd., Prague, Czech Republic).
Cells containing only pLuxAB were injected as a negative control to determine if the substrates supplied by the luxCDEfrp genes in the pLuxCDEfrp plasmid were capable of being scavenged from endogenously available stocks within the host in the presence of the luciferase dimer formed by the products of the luxAB genes on the pLuxAB plasmid.
Cells were then washed with 1 × PBS, and caspases 3, 8 and 9 activities were determined by using AFC-conjugated substrates supplied by Bio-Rad Laboratories (HerCAles, CA, USA), as previously described.
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After that, the slides were incubated with antidigoxigenin antibody conjugated to peroxidase, which was used to label the incorporated digoxigenin-labeled nucleotides, and added with the substrate supplied by the manufacturer.
The aluminium flux towards the substrates was supplied by an additional ring-shaped aluminium electrode mounted all around the circular magnetron.
The Mca-PLGL-Dpa-AR fluorigenic substrate was supplied by Bachem.
The enhanced chemoluminescence system (SuperSignal West Pico Maximum Sensitivity Substrate) was supplied by Pierce.
The enhanced chemiluminescence system (SuperSignal West Pico/Femto Maximum Sensitivity Substrate) was supplied by Pierce (Bonn, Germany).
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