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However, intracellular metabolite labeling following growth on labeled and unlabeled mixed substrates revealed a hierarchy in disaccharide catabolism in the presence of glucose: (i) complete inhibition of cellobiose catabolism, (ii) minimal catabolism of maltose, and (iii) unbiased catabolism of sucrose.
A competitive GnT-III inhibition assay using the synthetic substrates revealed a vital role for the Manβ(1 4 GlcNAc moiety.
The Y561-dependent substrates revealed a set of putative SRC substrates and subsequent bioinformatics analyses of these targets in human tumor datasets identified potential biomarkers of SRC activation in human tumors.
SEM examination of 2D substrates revealed a flat surface with a homogeneous pore size distribution (0.51 ± 0.03 μm) (Fig. 1A).
Capillary zone electrophoresis (CZE) analysis, of fluorescently labeled substrates revealed a range of hemicellulase, cellulase, pectinase and mannanase activities in the A. nidulans cultures (Data not shown).
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Biotransformation assays with different substrates reveal a successful immobilization of the d-Carb with similar specific activities, whereat the substrate NCPheAla was converted with the highest specific activity of 12.91 U/mg.
A comparative analysis of those spectra for the PEDOT PSS system and the hybrid films deposited on the silicon substrates reveals a decreasing transmittance of the hybrid films, which has to be caused by the additional absorption and scattering of light by the nanotubes and the PS nanoparticles (see Figure 1).
The low-magnification cross-sectional transmission electron microscopy (TEM) image (Figure 4a) of the ZFO thin film grown on the YSZ substrate revealed a dense and flat film with no macroscopic imperfection; the total thickness of the ZnO layer was approximately 125 nm.
Examination of the pH-sensitivity of the proteolytic activity using midgut samples collected at 12 h ABF and separated in polyacrylamide gels co-polymerized with protein substrate, revealed a pH optimum of 8, while no qualitative differences in proteolytic activity was detected at the various pH values tested (Figure 3).
Data extracted from cell tracking analysis of cells on 1D-substrates revealed a similar behavior upon CIL to those previously described for cells in 2D-conditions (Carmona-Fontaine et al., 2008).
X-ray structures of CTX-M enzymes with and without substrate revealed a narrow active site that expands significantly due to a conformational change upon cefotaxime binding.
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