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The chemical properties of the substrates processed by different treatments at experiment conducted at Spain (Lazcano et al. 2008) are summarized in Table 4.
These free oligosaccharides provide a biochemical record of endogenous substrates processed by ERAD-L (L, luminal).
We then used the fluorescence-assisted whole-cell protease activity assay in a screening procedure to isolate library members harboring substrates processed by TEVp and thereby identify its substrate profile.
What are the specific polysumoylated substrates processed by Wss1?
Substrates processed by human cell-free extracts can be scored by restriction enzyme cleavage to evaluate the repair of dI.
Manual inspection of the miR/miR* sequence reads comprising putative miRNA duplexes revealed that 70% of these miRNAs exhibit expected 1 2 nt 3'-end overhangs, the signature of substrates processed by the RNAse III enzymes, Drosha/Dicer.
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From the crystal structure it was predicted that flap substrates are processed by recognizing first a nick before processing, however the forked substrates are absent of a nick suggesting processing short flaps by tracking [ 8].
We show that non-amyloid substrates are processed by β-secretase in an endocytosis-independent manner.
Indeed, vertebrate pri-miRNAs, which are essentially stem-loop structures similar to Rnt1p substrates, are processed by paralogues of RNase III, to mature into effective guides for RNA degradation and translation repression[34].
None of these mutant substrates were processed by RSV integrase at a detectable level compared to wild type.
In this case, the objective was to build a multi-enzymatic biochemical 'AND' gate, made of three enzymes, in which two input substrates are transformed into one detectable product (one substrate being processed by one enzyme, the other by another enzyme).
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