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Substrates prepared from small- and large-grained polycrystals and single crystals were used to measure equilibrium and metastable contact angles and estimate the surface tension and equilibrium contact angle of the solid liquid interface.
We used data from at least three independently performed experiments and substrates prepared from three BHS, two domestic sheep, two mink and one white-tailed deer.
Dilutions of CWD brain homogenate in substrates prepared from human brain, transgenic mouse brain, and 239F cells expressing human PrP (129M or 129V) were compared for their ability to support amplification.
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Subsequently, an insoluble substrate prepared from BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium; TCS Cell Works) was added.
Less reassuring is the finding that there is no absolute barrier to the conversion of human PrPC by CWD prions in a protocol using a single round of PMCA and an entirely human substrate prepared from the target organ of prion diseases, the brain.
In this work, the cubic textured Ni 7 at.% W substrates were prepared from different powder metallurgy ingots by rolling-assisted biaxially textured substrate processing.
Substrates were prepared from four different types of carbon fibers, two with nanoscale diameters (nanophase, or less than or equal to 100 nm) and two with conventional diameters (or greater than 100 nm).
The PLGA membranes used as substrates were prepared from a 1.5 wt% PLGA dichloromethane solution by evaporation of the solvent on a teflon plate.
For magnetic tweezers experiments, DNA substrates were prepared from pRSgap (Stanley et al, 2006) into which a 1.4 kbp PCR fragment from pSFVI (3780 5140 bp) carrying a single EcoR124I site was cloned between the XhoI and the HindIII sites.
Substrates were prepared from ovine brain tissue from a VRQ/VRQ sheep (PG1521/10) and an ARQ/ARQ sheep (PG0220/11) and were used for testing VRQ/VRQ-derived and ARQ/ARX-derived milk samples respectively; both brains were from sheep from the scrapie-free flock, which tested negative for TSE by ELISA (Bio-Rad TeSeE, Bio-Rad Laboratories).
The brain homogenates used as substrates were prepared from either frontal cortex of non-CJD patients, or humanised transgenic mouse brain, with compatible PRNP-codon 129 genotypes (VV in the case of VPSPr and sCJD VV2 subtype) and MM for both cases of GSS).
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