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We present a novel technique to examine cell cell interactions and directed cell migration using micropatterned substrates of three distinct regions: an adhesive region, a nonadhesive region, and a dynamically adhesive region switched by addition of a soluble factor to the medium.
Substrates of three organic levels were created by adding different amounts of powdered fish feed (0, 5 and 10 g/l of sediment, respectively) to homogenized sediment and incubated with and without plants.
This was an open-label, single-sequence, repeat-dose study (CCX114671; ClinicalTrials.gov identifier NCT01489943) designed to evaluate the effect of repeat doses of vercirnon on the PK of probe substrates of three CYP enzymes and two transporter proteins in healthy subjects.
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Coatings were deposited on substrates of two different surface roughnesses, in order to test the ability of this coating system to function on rougher substrates.
The broad substrate specificities of the PhaJ4 and PhaC1 STQK) enzymes allowed for the production of homopolymers with strict control over the repeating unit composition from substrates of four to twelve carbons in length.
Substrates of two different resistivities in the range of 1 to 5 mΩ cm and 8 to 12 Ω cm where etched using the conventional anodization setup, resulting in two different pore morphologies.
In addition, we evaluated the quality factor Q of the configurations on substrates of two different thicknesses at a specific value of L in a more intuitive but computationally consuming way to verify QFP.
The results show that CyanAs can be divided into four groups, suggesting that they are substrates of four different enzymes.
We decided to test the substrates of five novel predicted transporters NCU01132, NCU01633, NCU05627, NCU05853, NCU05897, using heterologous expression of each transporter in the hexose-null yeast strain EBY.VW4000.
Beta-amyloid in AD is the result of the proteolytic metabolism of APP, an integral cell membrane glycoprotein of 697-770 residues which is the substrate of three proteolytic enzymes in two alternative pathways mutually in equilibrium [ 6].
Cleavage of poly-ADP-ribose polymerase (PARP), a downstream substrate of caspase-9, was markedly reduced in IMPDH2-overexpessing cells.
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