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We have identified >50 potential IAP substrates of both cytosolic and mitochondrial origin that bear hallmark N-terminal IAP binding motifs.
Limited proteolytic digestion was employed to provide additional support for formation of the dead end complex and to estimate the Kd values for substrates of both the forward and reverse reactions.
An increasing number of proteins have been identified as the substrates of both modifiers (Hunter and Sun, 2008; Ulrich, 2005).
Although the acceptor substrates of both enzymes differ in composition and glycosidic linkage of the Neu5Ac residues, both are large polymers.
Employing this technology, we were able to determine the consensus peptide sequences for substrates of both c-Raf and Mitogen Activated Protein Kinase Kinase Kinase 8, two highly homologous kinases with distinct signalling roles in cellular physiology.
We also observed that hypoxia induced strong acetylation at lysines 5, 8, 12, and/or 16 of histone 4, which represent acetylation substrates of both p300 and SRC-1 [22], [22], in Hep3B/shscx cells but not in Hep3B/shp300 cells (Fig. 5C).
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These results taken together provide evidence that Bcl-w may be a substrate of Akt both in vitro and in intact cells (Figure 4B).
Recurring patterns of neural activity, a potential substrate of both information transfer and transformation in cortical networks, have been observed in the intact brain and in brain slices.
We conclude that MPA is a substrate of both MRP2 and MRP4, but MRP2 is the main transporter involved in renal MPAG excretion.
Microscopic observations show normal cellular behavior when PANI-Cys is used as a substrate of both cell lines (HaCaT and LM2) as when they are cultured on TCP.
In the presented fMRI study, we adopted a standard two-phase ideomotor learning paradigm; a mixed between/within-subjects design allowed us to assess the neural substrate of both, IRA and ideomotor learning.
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