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Those differences distinctly affect SERS intensity measured for probe molecules: pyridine and sodium 2-mercaptoethanesulfonate. SERS substrates obtained using evaporation technique are ca. 1.5 times more efficient than substrates prepared using magnetron sputtering.
In vitro cell proliferation and cell morphology studies using MG-63 osteoblastic cells were carried out on the HA coated substrates obtained using the two deposition techniques, with untreated titanium grade 5 (Ti–6Al–4V) substrates utilised as a control.
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The resultant FADH− anion shifts to the IN position due to the positive electrostatic field near the IN position (state 5); the structure of pHBH with reduced flavin and bound substrate (obtained using anoxygenic solutions) represents this state (27).
As a result, the inferred substrate specificity obtained using truncated substrates remains dubious given their significant deviation from the native chain intermediates.
As input for this procedure the optical constants of individual layers of TiAlN, TiAlON, and Si3N4, deposited on Si substrates were obtained using spectroscopic ellipsometry and reflectance spectra for each material were calculated by the transfer matrix method using the obtained optical constants, showing good agreement with measured spectra.
For example, a fast (a few hours instead of 14 days with SBF) biomimetic deposition of CDHA coatings on Ti6Al4V substrates was obtained using a slightly supersaturated Ca/P solution with an ionic composition simpler than that of SBF.
The recombinant protease was assayed with 0.1-10 0.1-10azocasein in 50 mg/mlis buffer (pH 7.5) contazocaseinmM Co2+ at 42°C for 10 min. Kinetic parameters, such as Km (mg/ml) K cat (min-1) and Vmax (U/mg-protein) for substrates were obtained using Line-weaver Burk plot.
Kinetic parameters for both substrates were obtained using the Lineweaer Burk method.
Crack-free coatings with metallurgical bonding to the substrate are obtained using 0.6 mm initial powder thickness.
The lipase activity of the sample solution from crude extract using pNPL as substrate was obtained using the equation below.
These results demonstrate that the peptide substrate selectivity obtained using C5-Alk differsignificantlyly from that observed with C10-Alk and C15-Alk and suggests that this shorter probe is not suitable for proteomic analysis of farnesylated proteins because its peptide specificity profile is likely to be different than that for FPP.
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