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When the material released, it also leaves behind residue, so the original substrates may not be reused.
However, the cleavage of several substrates may not only contribute to increased tissue damage, but may also play an active role in disease progression.
However, some of these substrates may not be universally important for the arthropod-symbiotic lifestyle of these bacteria.
Since fixed substrates may not be under true saturating conditions, both Michaelis constants and turnover number determined in this study must be interpreted as apparent kinetic constants.
However, the experiment performed by Fahmy et al.[ 5] with soy protein and wheat gluten suggests that enzymatic cross-linking of substrates may not be necessary to produce a strong binder.
If the adverse effects are related to off-target substrates, γ-secretase modulators, which do not act at the active site of the enzyme complex and do not interfere with cleavage of non-APP substrates, may not carry these risks.
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The identification of Mn carbonates in these systems has important implications for the design of Mn treatment systems in that the provision of a carbonate-rich substrate may not be necessary for successful Mn precipitation.
Indeed, it has been suggested that although a complete pathway for a particular substrate may not exist in a single organism, partial and complementary pathway segments may exist in different organisms [32].
Vast areas of hard substrate may not be required in all areas, as small cobbles and shells may represent attachment substrata in the early stages of reef development [45] but this often depends on environmental requirements being met in the region and that sufficient larval supply is present.
At a more practical level, this result contains an important lesson for future compound screening campaigns: whereas it is commonly considered desirable to utilize the most reductionistic arrangement possible, our results suggest that screening with a single substrate may not be ideal in the case of IDE.
Nevertheless, this tetra-peptide substrate may not adequately represent the p27Kip1 protein.
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