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According to the well known metabolic pathways of E. coli, the hydrogen production from different substrates is dependent on the membrane-boundary formate-hydrogen lyase (FHL) enzyme complex.
The necessity of ATP for the binding to substrates is dependent on kinases.
One report provides evidence that when nuclear extract is prepared from cells infected with adenovirus, in vitro splicing of some substrates is dependent on the presence of U2AF65 [48]; however, splicing of other substrates can occur in the absence of U2AF65.
We further demonstrate that the phosphorylation of predicted substrates is dependent on CDPK1 both in vivo and in vitro.
The hydrolytic catalytic efficiency of some PON1 substrates is dependent on the single nucleotide polymorphism (SNP) Q192R (Li et al. 2000).
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The experimental results indicate that the crystalline structure and optical and electrical properties of ZnO thin films grown on PES substrates are dependent on the deposition temperature.
This study proves that the choice for an enzyme preparation to efficiently degrade a natural xylan rich substrate, is dependent on the xylan characteristics and could not be estimated by using model substrates.
The spreadability of filler metal on the substrate is dependent on one of two factors: mutual dilution between the metals or the formation of intermetallic compounds (Feng et al. 2012b).
The first system is represented by the firefly luciferase (FFluc) from Photinus pyralis which uses D-luciferin (a benzothiazole) as substrate, is dependent on ATP and results in the production of yellow-green light (557 nm).
It was also demonstrated that the number of particles settled on certain substrate is dependent not only on the shooting distance and the physical and chemical properties of particles but also on the material of the substrate [ 14].
These results demonstrate that the VRPR-AMC is a substrate for LmjMCA as for the Arabidopsis thaliana MCA AtMC9, that cleavage of the substrate is dependent on the catalytic activity of LmjMCA and that this activity is not inhibited by Z-VAD-FMK or E64, known inhibitors of caspase and cathepsin L, respectively.
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