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The ability of Skp to prevent the aggregation of model substrates in vitro is independent of ATP.
Directional growth and differentiation of adult rat hippocampal progenitor cells (AHPCs) were investigated on micropatterned polymer substrates in vitro.
The objective of the present work is to understand the vertical electric field stimulation of the bacterial cells, when grown on amorphous carbon substrates in vitro.
As seen with Rpt6-S120 and Rpn6-S14, phofphorylation of Rpt3-T25 also upregulates proteasome activity towards peptide and folded protein substrates in vitro and in cells.
Nine phosphoketolase candidates were expressed in S. cerevisiae of which seven produced significant amounts of acetyl-phosphate after provision of sugar phosphate substrates in vitro.
We expressed AAE15 in insect cells and demonstrated its AAS activity with medium chain fatty acid (C10 C14) substrates in vitro.
The environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo.
These data demonstrate that ILK directly phosphorylates physiologically relevant substrates in vitro.
Although the details of pupylation are unclear, PafA is sufficient to ligate deamidated Pup to substrates in vitro [10].
Our results demonstrate that PKA activates human HSL against lipid substrates in vitro primarily through phosphorylation of Ser649 and Ser650.
Several studies have demonstrated that recombinant PINK1 can undergo autophosphorylation as well as phosphorylate generic substrates in vitro [18] [20].
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