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Experiments performed using these substrates have provided unique insights into the roles of cell-substratum adhesion, cell shape, and ECM composition on important cell functions, including survival, migration, neurite outgrowth, and development of polarity.
Reactions of vaccinia topoisomerase and the tyrosine site-specific recombinase Flp with methylphosphonate (MeP) substituted DNA substrates, have provided important insights into the electrostatic features of the strand cleavage and strand joining steps catalyzed by them.
Crystal structures of EPKs bound to peptide substrates have provided insights into substrate recognition and specificity [ 11, 12].
Several crystal structures of AlkB and homologues bound to alkyl substrates have provided detailed information on their substrate recognition and repair specificity.
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Our kinetic analysis of primer-extension with gNTP substrates has provided some insight into the particular problems that prevent efficient Therminator-catalyzed GNA synthesis.
Recent work measuring activity of cloned Arabidopsis and yeast N-myristoyltransferases against synthetic peptide substrates has provided the opportunity to construct an improved, plant-specific myristoylation prediction algorithm.
Whilst studies on soluble substrates and substrate analogues have provided a wealth of information, understanding the mechanism of degradation of the natural substrate, crystalline cellulose, remains a great challenge.
In addition, the FSC-RHB substrate may have provided better plant anchorage and nutrient uptake hence the more chlorophyll content found in the leaves.
This crystal structure of the inhibitor complex and the MD simulation of the substrate complex EcDHNA DHNP have provided important insights into substrate binding and catalysis.
Several studies have demonstrated the influence of surface properties (surface energy, composition and topography) of biocompatible materials on the adhesion of cells/bacteria on solid substrates; however, few have provided information about the effect of the atomic arrangement or crystallinity.
In the evolution of complex molecular pathways the additional USP genes generated by WGD or SSD events could have provided substrate material for neofunctionalization or subfunctionalization.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com