Because substrates and ABPs have highly distinct mechanisms of action, a direct comparison of these two classes of agents in a relevant biological system would be valuable for understanding the strengths and weaknesses of each method.
Although these data do not support a model in which acidic and nonacidic GSMs have a differential effect on processivity that is mediated by charged residues at the end of the substrate's ectodomain, they indicate that both classes of GSMs have highly substrate selective effects.
We note that these two biofilm experimental systems are quite different, and that C. albicans adhesins exhibit high substrate specificity, even when they have highly related sequences in their binding domains [25].
The ADAMTSs have highly selective substrate specificities, seemingly associated with the noncatalytic domains of these enzymes, as exemplified by ADAMTS-4.
Among these parameters, the enzyme concentration (p-value = 0.0016) and substrate concentration (p-value = 0.0347) have highly significant effects (according to their small p-values) on the synthesis of wax esters as compared to the temperature and the alcohol/palm stearin molar ratio.
At the same time, models generalized for the entire human proteome may not perform well with CYPs, as these enzymes have highly variable regions – substrate recognition sites (SRSs) – that cannot be recognized as critical functional spots by evolutionary based methods.
They have highly variable protein protein interaction domains at the C-terminus, which confer substrate specificity for ubiquitination.
Following pattern transfer, the substrate surface now had highly ordered nanopatterned surfaces with desired shape and orientation [50].
The most highly connected substrates have more than a hundred and up to 841 connections (h+, h2 o, atp, pi, adp, ppi, nad, nadh).
These experiments showed that although simple, the agar plate-based assays using CPH substrates have the capacity to be highly information-rich.
