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After collection, substrates from each stage were weighed; particles were removed by scraping with a spatula and stored at -80°C in vials.
The fact that substrates from each of the three groups (A, B AB) are genuine, in vitro SETD6 substrates indicates that the two detection methods are complementary and thus both can be used to screen for new PKMT substrates.
When comparing solubilized xylan yields from enzymatic hydrolysis of pretreated solids at the 10 g glucan/L employed for evaluating substrate breakdown by enzymes, it should be kept in mind that compositional variations in the substrates from each pretreatment led to quite different ratios of enzyme protein to xylan content.
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Subsamples of substrate from each pot were retained for characterization and for determination of initial HS levels.
A measurement of the parchment substrate from each page was taken and spectra from the colored surface and natural parchment were overlaid.
A measurement of the paper substrate from each page was taken and spectra from the paint and paper were overlaid in order to ascertain which elements derived from each component.
Those are the successive ionic layer adsorption and reaction (SILAR) method and the successive under potential deposition (UPD) method, in which Cd and S are separately deposited on a polycrystalline Au substrate from each solution.
Approximately 10 mg of substrate from each substrate preparation was diluted in 400 μL of 50 mM sodium acetate buffer pH 5.5, and 100 μL of the N. crassa secretome was then added.
Using precleaned, silanized, and preweighed aluminum foil substrates, we divided the four sets of ELPI substrates obtained from each smoking machine session, using a true or smoothed puff profile, into fine (0.093 < aerodynamic diameter ≤3.930 μm, nine substrates) and ultrafine (aerodynamic diameter ≤0.093 μm, four substrates) particulate fractions.
Fifteen SERS spectra were collected from three substrates for each of the eight rotavirus-positive samples and the mock-infected MA104 cell lysate negative control to serve as multivariate biological fingerprints.
The cause and significance of this behavior are unknown, but it would seal the base of each ring, preventing even small substrates from crossing internally between rings.
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