Cell seeded substrates displayed a significantly higher elastic modulus than unseeded controls similar to native tissue.
Chondrocytes grown on pBC substrates displayed a higher viability compared to growth on the surface of unmodified BC substrates.
More importantly, mouse fibroblast NIH/3T3 cells cultured on these substrates displayed a more elongated cell morphology and had substantially higher cell densities than cells seeded on control PCL substrate, which indicates that introduction of U-DPy moieties into polymer matrixes could be used to create tissue culture surfaces that enhance cell attachment and proliferation.
MSCs in 3D substrates displayed a smaller area and stellate morphology, with cytoplasmic extensions that penetrated into the pores.
NatA, the major eukaryotic NAT in terms of targeted substrates, displays a broad substrate specificity profile by Nt-acetylating Ser-, Thr-, Ala-, Val-, Gly- and Cys- starting N termini of which the initiator methionine (iMet) was removed by the action of methionine aminopeptidase (1, 18, 19).
Early progenitor cells placed on 2D substrates displayed a characteristic blebbing morphology that can also be observed in early blastula stage embryos in vivo (Diz-Muñoz et al., 2010).
We demonstrate a set of individual holographic gratings in a thick substrate displaying a high contrast in diffraction efficiency as a function of the polarization of the read beam.
The Fe Al/steel substrate interface of the Fe2Al5 phase, after removal of the steel substrate, displayed a columnar structure growing toward the steel substrate, implying that Fe2Al5 grew at a rapid rate along the diffusion direction.
The substrate displays a "plug-into-socket" binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin.
The individual cells cultured in contact with the –COOH-substrate displayed a smaller spread area at day 1 and 3(l)) than those on –NH2- and –OH-modified surfaces, with strong evidence of focal contact formation at both days 1 and 3 and 3(p)).
