However, moving and foraging in this milieu creates significant mechanical challenges related to the presence of substrates differing in their size and orientation.
With the test conditions remaining the same, SERS signals were measured from the R6G dye applied onto GNR-Si and GNR-OPC substrates differing in thickness of the opal-like film.
DOI: http://dx.doi.org/10.7554/eLife.08716.006 To characterise the specificity of the disintegration reaction in more detail, we examined SsoCas1 activity for a variety of substrates differing in the nature of the 5′-flap or duplex arm released by the reaction.
However, residues that recognize substrate differ in each member.
Each substrate, differing in the DNA spacer length, was PCR-amplified with flanking primers and cloned into the BglII/SpeI sites of the yeast vector pCP5.1 (Christian et al. 2010) to create the TP series of plasmids (TP5-TP34) for the yeast activity assay.
If two substrates differ only in their catalytic rate of ubiquitin transfer, they will have different timing of degradation onset (in both one- and two-substrate systems).
If two substrates differ only in their binding affinity for APC/CCdc20, then each substrate by itself (in a one-substrate system) may not have dramatically different degradation onset timing.
