It should also be noted from Figures 2b and3a that the reflection spectra of the MNPs deposited on the glass substrate differ from those fabricated on thin a-Si films.
The pattern of pullulanase action against pullulanase substrates differs from that for α-amylases, indicating that other amino acids in addition to the three residues mentioned above are involved in catalysis.
The fact that both the SOFA-HDV-Rz68 targeting the HCV strand, and -Rz169− targeting the HCV strand, each of which possesses a cytosine in this position and exhibits a relatively high level of cleavage activity, suggests that the requirements for the efficient HDV ribozyme targeting of small substrates may differ from those of the SOFA-HDV ribozyme targeting long RNA molecules.
Aside from deacetylating histones, sirtuins are also active on non-histone substrates and differ from other histone deacetylases in that their activity is NAD+ dependent.
The presented in vitro experiments were all performed with CHO-derived mAbs as substrates, which differ from their plant-made counterparts with respect to their N-glycan structures.
Lignin inhibition within lignocellulose substrates will differ from these results, as in this study lignin was added to the solution and was not located throughout the lignocellulose structure.
Since biologically relevant substrates might differ from theoretical activities inferred from in vitro experiments – ' just because it can, does not mean it does' – the best method of substrate discovery is to identify protease-cleaved substrates in complex milieus (López-Otin and Overall, 2002; Tam et al, 2004).
However, the substrates differ in biodegradability rate.
Depending on the residual properties of the mutated proteins, interactions with partners, or the availability of the substrate may differ from natural heterozygosity, where both protein types cohabit since the beginning of their expression.
Thus, MCA have substrate specificities that differ from metazoan caspase usual substrates and are likely not to be accountable for recognition of caspase substrates or inhibitors as reported in several studies.
