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While both dsDNA and ssDNA substrates can potentially be used for recombineering, we generally favor 200 bp dsDNA when generating deletions or adding tags using BRED.
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Through a wafer bonding technique, the substrate is transferred into a highly thermal conductive silicon substrate to provide great thermal dissipation, and this new substrate can potentially provide a platform for light-emitting devices to achieve high brightness operation.
By simply cleaving apart or adjoining flurophores and quenchers on oligonucleotide substrates, one can potentially construct reagentless rizozyme or aptazyme chips that could simultaneously report the concentration of multiple different analytes.
Under illuminated anaerobic conditions, PNS bacteria can grow photoheterotrophically using organic substrates and can potentially produce H2.
In this way, the NWs are used independently from their growth substrate, which can potentially be recycled.
Our results demonstrate that ERAP1 has strong internal sequence preferences for the peptide-substrate that can potentially strongly affect peptide trimming rates.
However, it has earlier been reported [32, 33] that compounds that resemble substrates of FLuc can potentially function as competitive inhibitors of the enzyme thereby resulting in counterintuitive phenomenon of signal activation.
Caffeic acid O-methyltransferase (COMT) is an O-methyltransferase that tends to be broad in substrate affinity and can potentially act in various branches of the phenylpropanoid pathway.
In addition to revealing insights into the mechanism of self-cleavage, several aspects of our substrate specificity studies can potentially help direct future efforts to develop small molecule probes of the SOS pathway.
In addition, mushroom spent substrate can be potentially utilized in crop organic production (Lopes et al. 2015).
Our results indicate that the thiol-terminated substrate can be potentially useful for restoring the fouled surface, as well as maximizing the effective usage of the substrate.
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