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The expressed NAAAR racemized various substrates at an optimal temperature of 60 °C and had Km values of 24.8 mM and 12.3 mM for N-acetyl-d-methionine and N-acetyl-l-methionine, respectively.
By growing LaAlO3 films on SrTiO3 substrates at an oxygen pressure of 37 mTorr, sufficiently high to alleviate oxygen deficiency in SrTiO3, we show that the properties of the 2D electron gas at the LaAlO3/SrTiO3 interface are in quantitative agreement with the electronic reconstruction mechanism.
Optical microscope images of (a) 80 nm, (b) 180 nm, and (c) 250 nm on PDMS substrates at an identical strain of 50%.
Alumina/zirconia (Al2O3/ZrO2) multilayer thin films were deposited on Si (100) substrates at an optimized oxygen partial pressure of 3 Pa at room temperature by pulsed laser deposition.
The SiO2 layers were deposited on the unheated plastic substrates at an rf-power of 100 W, gas mixing ratio of Ar/O2 of 4/5, and total pressure of 3.5 mTorr.
In order to investigate the influence of the substrate used for enzyme production on hydrolysis, the F-1663/S F-1663/SWS and F-1663/WSenzyme supernandnts F-1663/B F-1663/Bmixturenzyme usupernatantscand thehydrolyze the three substrates at an enzyme loading of 3 FPU (filter paper unit)/g WIS.
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This process is readily scalable, and the devices can be fabricated on large substrates at a fraction of the cost of traditional micro-fabrication methods.
KSI binds substrates at a surface cleft, TIM binds substrate at an exposed 'cage' formed by closure of flexible loops; and, DAP epimerase binds substrate in a tight cage formed by an 'oyster-like' clamping motion of protein domains.
Substituting alanine or serine resulted in mutants that excised substrates at a faster rate than wild-type.
The films were deposited on steel substrates at a constant temperature of 300 °C by r.f.
A PEDOT PSS layer was spin-coated onto the ITO substrates at a speed of 4000 rpm for 40 s and then baked at 150 °C for 10 min in air.
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