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The autocatalytic route was created using AgCl similar to PdCl2 to develop calcium phosphate layer on Ti6Al4V (TAV) substrates as previously demonstrated by the present authors.
kobs and Hill coefficients were determined from the cleavage kinetics of [P]-labeled RNA substrates as previously described (Korennykh et al., 2009).
Phagocytosis by Sertoli cells was measured, using a specific assay that discriminates between bound and ingested phagocytosis substrates as previously described.
PfCK2α autophosphorylation generated a band of ~60 kDa in size, but the dead mutant did not autophosphorylate, and was unable to phosphorylate exogenous substrates, as previously reported [ 19].
To determine in-gel complex I activity, assays of NADH dehydrogenase activity were performed in-gel with NADH and nitrotetrazolium blue (Sigma, Poole, UK) as substrates, as previously described (Calvaruso et al., 2008).
The probes were modified at the 5′ end with a poly (T 10-poly (C)10 T 10-polyaC 10tail toe attachment to the plastic substrates as previously described by Dufacilitate [ 20].
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The putative SRC-dependent proteins include known SRC substrates as well as previously unrecognized SRC targets.
The activity was measured by spectrophotometric method with p-nitrophenyl α-l-arabinofuranoside (pNPA) (Gold Biotechnology, St Louis, MO, USA) as substrate, as previously described (Amore et al. 2012).
AMPK activity was determined using the SAMS peptide as an AMPK substrate, as previously described [22].
Phosphatase activity was assayed using a 32P-labeled glycogen phosphorylase a substrate, as previously reported [2].
Elastase activity was determined in a spectrophotometric assay using elastin-Congo red (Sigma) as a substrate, as previously described [49].
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