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Mixotrophic growth, in which organic substrates are fed alongside H2/CO2, alleviates the ATP limitations and thus improves the yields for both aerobic and anaerobic butanol production.
Bacterial diversity has been found to vary at the anode of the MFCs when various different substrates are fed.
Coherently, the acetate P/O ratio tends to prevail over that of propionate when both substrates are fed simultaneously.
In CLEs, C-enriched carbon substrates are fed to the cells and, driven by the cells' metabolic and enzymatic activities, isotopically labelled carbon atoms are incorporated into the metabolite pools [ 11].
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In this work, three hybrid vertical down-flow constructed wetland (HVDF-CW) systems with different compound substrates were fed with domestic sewage and their pollutants removal performance under different hydraulic loading and step-feeding ratio was investigated.
The total carbon uptake observed when both substrates were fed to this culture was approximately double that of the propionate or acetate uptake when the substrates were fed individually.
In acetate enriched cultures, the total VFA uptake capacity obtained with acetate feeding (0.73 C-mol/ C-mol.h)) was approximately equal to the sum of aC-mol/ C-molropionate uptake, when the substrates were fed simultaneously.
Fermentation was initiated with 8% unwashed corncob and the substrate was fed to 18.4% (w/w) at 24 h.
Inlet and outlet manifolds, also etched into the substrate, were fed by 1.4 mm inner-diameter tubing ports.
The first and second feedings with NH3-H2O2-pretreated and unwashed corncob were conducted at 12 h and 24 h; the substrate was fed to 8% and then to a final concentration of 16% (w/w).
The ethyl ferulate/soybean oil substrate was fed through the four, packed-bed, enzyme columns in series with each column fitted with a recirculation pump, which improved conversion and throughput compared to smaller, batch bioreactor systems.
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