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Glass flake (GF) substrates are defined as highly planar platelets with a very smooth surface.
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One activity unit (U) on these substrates was defined as the amount of enzyme that released of 1 μmol of p-nitroaniline under the given conditions.
Therefore, two groups of substrates were defined: (1) PCL nano-fibrous substrate modified with oxygen plasma for culturing of HDFs and (2) PCL unmodified nano-fibrous substrate for culturing OSTs and HDFs.
In a preliminary analysis, candidate substrates were defined as those compounds having one substructure involving a reaction centre and at least three substructures found in a reported substrate for a given enzyme.
One unit of activities using p-nitrophenyl substrates was defined as the amount of protein capable of releasing 1 mmol pNP from the substrates per minute.
A panel of 24 kinase-substrate interactions comprising 14 different protein kinases and 18 substrates was defined as the test set to evaluate the ability to replicate the protein substrate phosphorylation observed in solution on protein arrays (Table 1).
A set of 24 kinase-substrate interactions comprising 14 different protein kinases and 18 substrates was defined as the test set for benchmarking protein microarray results against solution phase assays.
The position and the arrangement of the tubes on the substrate are defined by UV photolithography structuring of the polymer film.
The particle deposition efficiency, distribution non-uniformity and average impact velocity on the substrate are defined in the study as three indicators for evaluating the deposition quality.
A substrate was defined as an individual coupon, polypropylene or wet sample.
The apparent adhesive strength between the fouling deposits and the substrate was defined as the work required to remove the deposits per unit area from the substrate.
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