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The proposed ink compositions for flexographic printing on paper substrates are based on an environmentally friendly acrylic binder and contain inorganic fillers with platelet particles incorporated in the polymer matrix.
The substrates are based on conventional photolithographically patterned copper clad polyimide (50 μm thick, Pyralux LF series, DuPont, NC) films whereby the copper squares (dimensions visible in the results) are coated with a low melting point solder through dip-coating.
ICB substrates are based on raw biomass samples or alcohol-insoluble residue (AIR) preparations which are standard crude preparations of polysaccharides widely used as the starting point for biomass analysis, for example, saccharification assays [ 33, 34].
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Therefore, by screening nonbiased peptide libraries we were able to identify optimal sequences that may have been overlooked had the design of the substrates been based on only currently known substrates, a strategy commonly adopted by fluorescent-based assays that prevents the detection of unknown activities.
The first series of PKC substrates is based, in part, on the Ca+2 indicators developed by Tsien and his collaborators during the 1980s.
The choice of substrates was based on their nominal oxidation state of carbon, which reflects the energy yield and the biogeochemical reactivity of a compound.
Furthermore, it must be noted that the annotation of the substrates is based on the available data at present, and therefore incomplete.
The sequence of the first three substrates was based on the Rnt1p cleavage signals found at the 3'-end of U2 snRNA because it was previously shown that this RNA could be cleaved even when the cleavage site is unpaired[9].
Production of ICB substrates was based on the same dyeing chemistry as for the CPH substrates, but the cross-linking step was omitted.
Our conclusion that CtpS assembly is repressed by substrates is based on Figure 1E, where the addition of substrates causes depolymerization and Figure 2E where addition of substrates in the presence of a glutamine analog causes depolymerization without repolymerization.
The assay for PFTase activity employed here using FPP and analogues 2 and 3 as substrates is based on the previously published assay by Pompliano et al. and later refined by the Poulter group.
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