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Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
Depending on the solvent, enzyme, substrates and time, as well as the addition of molecular sieves, it is possible to convert 100% of the sugar to a glycolipid (Amos et al. 1998), but in other systems the yield is lower (Ducret et al. 1996; Tarahomjoo and Alemzadeh 2003).
Such studies have begun to define distinct brain substrates and time windows for particular ASA subprocesses.
In this respect, its descriptive accuracy was verified using results from other authors (taken from the published figures by means of GetData Graph Digitizer 2.24), selected in such a way that they implied different methods, substrates, and time domains.
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The effect of viscoelastic substrates and time-dependent electro-mechanical coupling of piezoelectric materials on the deformed shapes is also studied.
Three crucial process parameters, namely inulin concentration (1 17.5 %), enzyme loading (1 10 U/g of substrate) and time of hydrolysis (0.5 23.5 h) were investigated to get maximum sugar yield.
An alternate procedure based on chronopotentiometric measurements achieved in particular conditions of substrate and time duration was proposed form which the kinetic rate constants, Km and Vm, could be directly deduced after numerical calculation.
Data from a subset of isolates with the highest activity for each substrate and time were standardized within each substrate and time.
> Kplant values changed in response to both type of substrate and time after last irrigation in S. officinalis samples (Fig. 4A, Table 4).
For instance, allosteric inhibitors have the potential to be noncompetitive or uncompetitive versus a particular substrate and time-dependent and to target sites in the enzyme other than the active site.
Gericke and Pinches [37] reported that the size of particles that form intracellularly could be controlled by altering key factors such as pH, temperature, substrate concentration, and time of exposure to the substrate.
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