Sentence examples for substrates and reagents from inspiring English sources

All the substrates and reagents used were commercially available and were used without further purification.

Substrates and reagents for enzyme determinations were NAD(P H, DTNB, GSH, GSSG, glutathione reductase, and tert-butyl hydroperoxide; all of them were purchased from Sigma-Aldrich (St .Louis, Missouri, USA).

This could be attributed to differences on the processing of IIF samples, namely substrates and reagents used for the detection and on the evaluation of the results, especially when antibodies of cytoplasmic specificities are present in the same serum [ 3, 4, 11].

ortho- t-Butyl carbonate (Boc) and pivalate (Piv) salicylic derivatives displayed a desirable reactivity profile over the greatest range of starting substrates and organometallic reagents.

In conclusion, these data indicate that substrates and secondary reagents used in immunofluorescence assays are determinant factors for the detection of ANEA.

For detection we used the biotinylated goat anti-mouse IgG BA2001 (dilution 1 100), a horseradish peroxidase avidin complex and AEC as substrate (secondary antibody and reagents from Vector Laboratories, Burlingame, CA).

The substrate and coloring reagent agaroses were supplemented with 0.5 mM EDTA and applied sequentially.

Luciferin substrate and stop reagent were purchased from Promega (China).

10  μL (20  μg) of total protein containing CS was added to test well containing reaction substrates reagent and 10  μL of negative control reagent was added to control well.

The objective of this global comparative field study was to evaluate the performance of rFVIIIFc with a variety of one-stage clotting and chromogenic substrate assay reagents and instruments in clinical haemostasis laboratories.

By contrast, the generation of micropatterns by photolithographic methods often requires a multistep process, hazardous reagents, expensive substrates, and processing equipment.