The mean background sugars were subtracted from the data for each substrate and data were again centered and scaled.
The kinetic parameters were determined using various concentrations of substrates, and the data were fit using the Michaelis Menten equation.
However, we also validated the technique by making a direct comparison between the performance of the CPH substrates and activity data determined by the PAHBAH method to measure the release of reducing ends [ 17].
We conclude that numerical comparison of FT-IRIS-derived parameters of tissue composition should account for substrate type and data collection mode, while analysis of overall tissue architecture may be more invariant between modes.
We compared baseline-corrected raw spectra for M. pneumoniae strains FH, M129, and II-3 to assess reproducibility, collecting five spectra from three separate wells on a 40-well array or three independently fabricated substrates (Figure 1A and data not shown), for a total of 15 spectra per strain.
Metabolic flux analysis involves the calculation (or estimation) of in vivo fluxes from substrate and product data, by using a system of linear equations developed from reaction stoichiometry [19].
Model results presented here likely overpredict the amount of suitable habitat in some areas because fine-scale bathymetric features (10's of metres), substrate and current data are not available.
Urinary NAG excretion was determined at 405 nm using p-nitrophenyl-N-acetyl-β-D-glucosaminide as substrate and the data were expressed as U/24 h [ 7, 15].
Mice were injected i.p. with 200 μl of a 15 mg ml−1 luciferin solution (XenoLight D-Luciferin K+ Salt Firefly; Caliper Life Sciences) in PBS, 5 min before imaging to allow uptake of the substrate and all data were collected as photons/second.
We have demonstrated that Fbxl7 harbors a molecular signature that recruits Fbxl18, identified the acceptor site for Fbxl18 activity within its substrate, and present data suggesting that levels of Fbxl18 affect the ability of cells to remain viable from Fbxl7-induced apoptosis.
