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pNP-based chromogenic substrates and complex substrates were used to assay the enzymatic activities of the fungal secretomes.
Moreover, the RKIP pathway has been well studied in [ 13, 21] by using the mass-action law to describe the quantitative relationships between substrates and complex.
Gel electrophoresis and carboxymethyl cellulose zymograms were performed as described elsewhere [ 49]. p-nitrophenol-based chromogenic substrates and complex polysaccharide substrates were used to assay the enzymatic activities of the fungal secretomes as described elsewhere [ 13].
The identity of VAD FMK-binding proteases, exact role of these proteases, specific target substrates and complex cascade of induction/actiVAD FMK-bindingproteasesin parasitexactmains to be elucidated.
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Multiple-dynamic effects of "bacteria-AQDS-DOM", which result from AQDS, shifted the microbial community and synchronously derived terrestrial DOM, which potentially changes the DOM substrate and complex formation of As III -Fe(II)-humic DOM.
The quantity of DNA immobilized increased with time of exposure, and the deposition rate and final amount deposited depended upon the properties of the substrate and complex.
For example, more elucidation is required on the exact stoichiometry of TatA proteins per substrate and complex before such information can be incorporated into iJL1678-ME.
The motion on the fast time-scale is dominated by the complex C decoupled from the substrate S. On the slow part, the changes of substrate and complex are balanced.
Note also that these equations are independent of ϵ p since the product-forming step is irreversible and hence the fluctuations in substrate and complex are necessarily decoupled from its fluctuations.
For each choice of initial conditions (concentration of substrate and complex), we report the largest error σ (in percentage) for which the resulting uncertain measurements allow to invalidate the Michaelis-Menten model.
In enrichment cultures to promote growth of lignocellulolytic microorganisms, biomass feedstocks are used as substrate and complex communities, such as those found in compost or soil are applied as inoculum [ 11, 16].
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