Sentence examples for substrates and analyzed from inspiring English sources
All supercritical extracts were from two commercial PR coated on two substrates and analyzed by UV absorption spectrometry.
To assay Plk1 and Plk2 kinase activities, immunoprecipitated or bacterially expressed and purified kinases were incubated either with immunoprecipitated proteins or 500 ng recombinant proteins from bacteria as substrates and analyzed as described previously [6].
In order to elucidate substrate-induced changes in cellulosome composition, we grew C. thermocellum on pretreated switchgrass and several other model substrates and analyzed the changes in subunit profile of cellulosomes isolated from cell-free broth using quantitative proteomics (15N metabolic labeling and LC-MS/MS).
The Hall measurements were carried out on carbon ion implanted sapphire substrate and analyzed.
The kinetic parameters Km/Kcat were determined using various concentrations of CMC as substrate and analyzed with Lineweaver Burk plot.
The mode of action of recombinant PoAbf62A was examined using sugar beet arabinan as substrate and analyzed by methylation and 13C NMR.
Finally, plates were washed, developed with 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidineh asBioTek plate reader at 450 nm.
To this end, Petri dishes with transformed Chlamydomonas colonies were exposed to the substrate and analyzed by luminescence imaging (Fig. 5).
Immunocomplexes were visualized using a NBT/BCIP substrate and analyzed in Molecular Imager ChemiDoc MP Imaging System with Image Lab 5.2 Software (Bio-Rad).
This approach is a refinement of the stretched sample method, in which MSI, or profiling, is conducted on tissue samples that are placed on an array of beads embedded on a Parafilm substrate and analyzed via MALDI MS. A similar approach has also been used for laser ablation electrospray ionization MSI.
Blots were developed using the SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) and analyzed on an Alpha Innotech Gel Imagining System.
