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Specific methanogenic activity (SMA) determines the methane-producing capability of the sludge for a specific substrate at the concentration level where the availability of substrate is not a limiting factor (Hussain and Dubey 2013).
It has been shown that the amount of the synthetase substrate is not a limiting factor for the production of the autoinducer [ 40, 41].
Comparison of cell-based and test tube assays suggests that PAM is operating at close to Vmax in cells – its affinity for the Fc-peptidylglycine substrate is not a major factor.
We had considered that the improved solubility of 1 and its product might result in faster turnover; however, the sluggish rate at which 1 is deformylated indicates that the solubility of the substrate is not a limiting factor in the rate of turnover.
Van der Land [3] also found that corals from the lagoon zone of Saba Bank were "small in size and number," but concluded that substrate was not a limiting factor for coral settlement.
"Fear is not a factor.
"This event is not a factor".
Poverty is not a factor.
Compensation is not a factor.
Time is not a factor.
"Control is not a factor.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com