Sentence examples for substitution library from inspiring English sources

Chromosome segment substitution library.

A high quality combinatorial library specifically customized to a given binding-interface can be rapidly designed by high-throughput mutational scanning of single substitution libraries.

The resulting specificity pattern is in good agreement with previous publications that used peptide substitution libraries to directly compare different residues in one position on the affinity or transport rates of murine TAP[19], [34], [36].

Briefly, codon substitution libraries for the amino acid 582 590 region of yeast Hsp90 (Hsp82) were transformed into the DBY288 shutoff strain (can1-100 ade2-1 his3-11,15 leu2-3,12 trp1-1 ura3-1 hop82::leu2 hsc82::leu2 ho::pgals-hsp82-his3) of S. cerevisiae by the lithium acetate method (Gietz and Woods 2002).

By all-around modifications on R1, R2 and R3 substitutions, consecutive library synthesis, and testing, we improved the aqueous solubility (5-fold improvement in solubility, from 0.1 to 0.5 μg/ml) and anti-cancer activity (10-fold improvement in EC50 from 0.72 0.98 μM to 0.08 0.16 μM) in the new lead thiazolidinone compound 31.

Produces a negative class of in silico passenger mutations by random nucleotide substitution in a library of expressed human mRNA transcripts from NCBI RefSeq, according to the distributions specified in the passenger mutation rate table (Supplementary Material ).

In two separate comparisons, we detected identical IgH sequences containing identical nucleotides substitutions in each library.

This is the result of building substitution matrices on libraries of sequences that are much more highly diverged than those within mammalian proteomes.

In these studies, mutations were generated by error-prone PCR and average base substitution rates from libraries of clones were evaluated directly by sequencing.

In two separate comparisons, we detected identical IgH sequences with identical nucleotides substitutions in each library (see Figure 7-figure supplement 3).

To overcome this problem, here we have used two different TNF mutant libraries, each containing random substitutions at six selected amino acid residues, and utilized a gene shuffling method to construct a randomized mutant library containing substitutions at 12 different amino acid residues of TNF.