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Arginine and lysine were substituted with light (Arg0, Lys0), medium (Arg6, Lys4), or heavy (Arg10, Lys8) amino acids (Cambridge Isotope Laboratories, Inc., UK).
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I am gratified, though somewhat surprised to discover that there is no strict need to use any dairy at all in a chocolate pot; Coady's spring water ganache is, as the name suggests, made with boiling water, though she suggests this can be substituted with a "light fragrant tea" or even Champagne "though I think it's a bit of a waste".
Heavy and light chains were produced in Escherichia coli and refolded with a conditional ligand, which was substituted with a peptide of interest upon exposure to UV light (1 h, 366 nm) [ 23, 24].
STAT3 fused with a tag for its biotinylation was expressed and biotinylated in HEK-293 cells, which were fully substituted with 13C-labeled arginine in place of the light arginine.
One was a variant of Antibody A (Antibody A-S) in which the N-terminus of the light chain had an aspartate substituted with a glutamate and the N-terminus of the heavy chain had a glutamate substituted with an aspartate; in essence, the two termini were swapped.
The resulting anilide, substituted with a strong electron-donating group, is a chromophore that absorbs light at 278 nm.
The light bars in these figures are the Poisson distribution from Eq. (1), where λ is substituted with the average cell occupancy of each cell concentration.
Jack Rodwell substituted with first-half injury.
In light of these statics, it is my assertion that "George Zimmerman's" name in the title of this essay can be substituted with that of any perpetrator of a racialized killing with the relevancy of the question remaining the same.
After 50 min of electrophoresis, 'dark cathode buffer' (Invitrogen) containing 0.02% Coomassie Brilliant Blue G-250 was substituted with 'light cathode buffer' (Invitrogen) containing 0.002% Coomassie Brilliant Blue G-250.
Compound 2 was substituted with 4-chlorobutyronitrile.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com