Non-template control (NTC) samples contained the same PCR reagents as above but had 2 µl of water substituted for template.
Non-template control (NTC) samples contained the same reagents as above for each of the 7 distinct PCR reactions but had 2 µl of water substituted for template in each case.
A negative control was set up by substituting the template with double-distilled H2O.
Ultrapure Milli-Q water was used for adjustment of volume and concentrations and as a template substitute in negative controls.
We considered avoiding this step by substituting a foam template, but Jane insisted on using the real thing.
The input template (substituted with filenames and URLs) is stored in a database field with a 64KB limit.
Bosentan analogs consist of pyrimidine as the central template substituted with four substituents at positions 2, 4, 5, and 6, with sulfonamide attached to the 4-position of the central pyrimidine (Supplementary Fig. 6).
A negative control, substituting water for template, was included in all PCR experiments.
Mimotopes can potentially serve as lead compounds in the development of low molecular weight substitutes of the template protein for use in drug and vaccine design [ 23].
PCR II (semi-nested): components, their concentrations, and thermal profile were similar to PCR I, except we used 2 μl of PCR I-product as template, substituted primer GIo by primer DRB1.2 [ 40], increased the annealing temperature to 65°C, and increased the number of cycles to 30.
