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The pattern of distribution of calnexin (CNX) and AKT, both previously identified at MAMs, further substantiated the specificity of c-FLIPL subcellular localization.
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It also substantiates the specificity of our assays as there is no apparent bias for selection of highly expressed ncRNAs.
Moreover, Rad51 foci do not form in cells lacking Brca2, CtIP or Rad51, substantiating the specificity of the assay.
To substantiate the specificity of PRLR staining results, we examined PRLR expression in the SCCHN cell lines PCI-6A and PCI-6B using immunoprecipitation.
To substantiate the specificity of the effect, MCF7 cells were transfected with the miR-744 and miR-663 inhibitors before the resveratrol treatment.
Moreover, co-electroporation of siRNA to Snail1 along with the full-length gene rescued the excessive myogenic differentiation caused by attenuation of Snail1 activity [see Additional file 8: Figure S8C,F,G], further substantiating the specificity of this loss of function approach.
It also substantiates the high specificity of our peptide-targeted system, and demonstrates little or no nonspecific uptake of nanogels by SK-OV-3 cells as shown by the constant levels of EGFR expression and unaltered chemosensitivity after nanogel treatment.
2) More work is needed for substantiating the target specificity of SfbI-TEDs and FbaB-TED.
To substantiate this observation and to ascertain the specificity of the blocking effect, we tested RBD-variants conthening the R59A mutation which lowers the affinity of RBD for Ras-GTP by about 30fold [ 14, 42].
Functional experiments substantiated the role of L-MSCs in normal lung development.
Several reviews however, substantiated the safety of Cimicifuga racemosa preparations [64, 65].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com