The significance of the microarray data was substantiated for selected examples by qRT-PCR and quantitative primer extension analysis.
All primary and secondary outcomes measured in the systematic review will be assessed using a validated or substantiated scale or tool, for example: Care giver Burden Scale, McGill Pain Questionnaire, Palliative Outcomes Scale and EQ-5D.
While most of these identified associations remain to be substantiated by further experiments (for example, in crossing designs or functional analysis), these data to date suggest that it is possible to identify QTNs in plants that explain a significant proportion of the phenotypic variance in natural populations.
It is believed to be only the fourteenth adequately substantiated example.
The theoretical results are substantiated with numerical examples.
The main conclusion, which is substantiated through application examples, is that robust and reliable estimation applications based on first principles models of considerable complexity, can be designed and implemented for use in an industrial environment.
This line of argument is substantiated by several examples of extreme polyploidy in giant bacteria.
In contrast, several studies have substantiated differences between these two populations; for example, there are higher odds of a family history of psychopathology and developmental problems in adolescents [ 11, 12].
However, additional biochemistry studies are needed to substantiate the biological relevance of SRM1, for example to verify if the Rad51-G143P mayant may exhibit other defects, such as in polymerization, ssDNA binding, or interaction with mediator proteins.
The Boston Marathon bombing, wherein tens of thousands of images and videos were contributed by citizens for analysis, presents a real-world experience to substantiate the above example [55].
