It also substantiates the specificity of our assays as there is no apparent bias for selection of highly expressed ncRNAs.
Moreover, Rad51 foci do not form in cells lacking Brca2, CtIP or Rad51, substantiating the specificity of the assay.
The pattern of distribution of calnexin (CNX) and AKT, both previously identified at MAMs, further substantiated the specificity of c-FLIPL subcellular localization.
Moreover, co-electroporation of siRNA to Snail1 along with the full-length gene rescued the excessive myogenic differentiation caused by attenuation of Snail1 activity [see Additional file 8: Figure S8C,F,G], further substantiating the specificity of this loss of function approach.
It also substantiates the high specificity of our peptide-targeted system, and demonstrates little or no nonspecific uptake of nanogels by SK-OV-3 cells as shown by the constant levels of EGFR expression and unaltered chemosensitivity after nanogel treatment.
2) More work is needed for substantiating the target specificity of SfbI-TEDs and FbaB-TED.
To substantiate this observation and to ascertain the specificity of the blocking effect, we tested RBD-variants conthening the R59A mutation which lowers the affinity of RBD for Ras-GTP by about 30fold [ 14, 42].
Moreover, the specificity of MEF2C silencing was substantiated by the lack of effects of siMEF2C on MEF2A, MEF2B or MEF2D, as well as on the unrelated proteins FAK, JNK, SHP2 and GAPDH.
Experimental evidences lucidly substantiate the effect of indirect interaction like DNA deformation and desolvation kinetics, in empowering ZFPs to accomplish partial sequence specificity functioning around structural properties of DNA.
