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This was due to S5′s substantially lower background fluorescence before activation.
We repeated the measurements with freshly prepared proteoliposomes, resulting in a substantially lower background.
Our data shows that sequencing of cytosolic RNA yields substantially lower background from immature transcripts, and we propose that cytosolic RNA-seq should be the method of choice for de novo transcriptome assembly and tissue specific expression profiling.
In the revised manuscript these are Figure 3 figure supplements 1 and 2. Background of uptake was not subtracted either in Figure 3 figure supplement 1 nor supplement 2. We repeated the measurements with freshly prepared proteoliposomes (new Figure 2C), resulting in a substantially lower background.
Similar(56)
Research had shown that children from low-income, minority backgrounds tested "substantially lower" than middle-class children in school-related skills, and that they continued to have educational deficits throughout school.
Vaccination uptake was substantially lower among girls from lower socio-economic backgrounds.
Eastern European professionals in all occupational categories tend to have substantially lower incomes than skilled workers from Northern and Western Europe despite their similar educational backgrounds.
Survival for the least affluent remains substantially lower than for the most affluent, even after adjustment for the differences and trends in background mortality among deprivation categories; without that adjustment, the observed difference in survival would be even greater.
"We substantially lower CO2 emissions.
Thus, installation costs are substantially lower.
(Zhukova says that the figure was substantially lower).
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