However, conventional PCR followed by Sanger sequencing, pyrosequencing, or HRM analysis may often fail to identify mutations in samples with a substantial wild-type DNA content [ 6, 13, 14].
Our in vivo studies on the role of SR-B1 during mycobacterial infection also revealed no substantial difference between wild type and SR-B1−/− mice when infected with either a low or a high dose Mtb or when investigated in the context of dietary cholesterol (Fig. 4D, Fig. 5B, Fig. 6E).
For abp1-1, abp1-1s or bsm mutants it was only possible to evaluate the BSM expression in the heterozygous plants (due to the lethality of homozygous mutants) and this showed no substantial differences compared to wild type plants (Dataset 1).
Further reduction in recovery times was not seen when all three tandem repeats harboured the mutation corresponding to N1168F in full-length CREBBP (P > 0.05), but a substantial increase in the wild type was seen with the addition of SAHA (2.5 ± 0.1 seconds; P < 0.05).
Fourth, while our criteria were stringent (significant and substantial differences from two different wild type strains), strain differences may account for some of the differences between wild type male and female and FruM over-expressor male and female strains, respectively.
Such a missing observation can for instance be due to knockouts or knockdowns exhibiting no substantial effect because of low wild type gene levels.
Hexamers with two WA or WB mutations showed substantial recombination activity (∼65% of wild type in 1 min reactions), whereas the mixed subunit population that gives hexamers with a single mutation (50%) showed at least wild-type activities after correction for the wild-type (25%) and double-mutated (25%) population.
We actually demonstrated a small population of melanoma cells harbouring activating BRAF mutations in a substantial number of tumours that were wild type by direct sequencing.
However, at ten and 12 weeks substantial MIN development is noted in wild type mice, compared to a greatly diminished incidence of MINs in NG2 null mice.
However, in our case, it is possible that the expression profiles of the long-lived mutants have substantial differentiation compared to that of the wild type.
Accordingly we observed substantial increases in ROS production compared to wild type when the ubiquinone synthesis mutant coq5Δ was treated with binaphthoquinones.
