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Cells showed significant viability at 24 h (98%) and slightly lower, but still substantial viability, at 48 h (72%).
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Dinitrophenylation and tyrosylation could be achieved without substantial loss of viability as measured by dye exclusion.
Mutants defective for PKC1 show a substantial decrease in viability when starved (Krause and Gray 2002).
Concentrations in excess of 5 μ M markedly reduced the recovery of all cell lines, indicating substantial loss of viability.
Only plastic species can adapt to the drastically changed conditions of life in captivity without a substantial reduction in viability and fertility.
Downregulation of UPF1 caused a substantial reduction in viability of cell populations in the three cell lines at 48 h and 72 h and 1(c)).
By contrast, treatment with isradipine resulted in substantial improvements in viability over a broad dosage range, resulting in ∼70% survival at 1 μM and 50% survival at 15 μM.
We transformed K12 E. coli expressing nicking Cas9D10A with plasmids expressing individual and paired sgRNA combinations and cultured in pooled transformations and determined paired nicking did not lead to a substantial reduction in viability (Supplementary Figure S7).
Both 4C7 (ED50=6.61±1.3 ng/ml) and 4C9 (ED50=7.86±1.53 ng/ml) caused a substantial decrease in viability when compared with rhTRAILWT (ED50=46.8±1.1 ng/ml), with the DR4-selective variants reaching cell death levels of 88 and 83%, whereas rhTRAILWT was only capable of 50% cell death induction at the highest concentration measured (250 ng/ml).
Collectively, these defects in SC differentiation lead to a substantial reduction in viability, which we suggest is caused by defective excretion, as animals become bloated with fluid and the tubules fail to respond to diuretic stimuli, producing only very low levels of secreted primary urine.
The repeated attacks on his insistence that Scotland and the UK should share the pound, in a formal currency union, and warnings about the decline in oil income from the Institute for Fiscal Studies and the UK government raise substantial questions about the viability of his proposals.
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