Your manuscript has been evaluated by Detlef Weigel as Senior editor, Jesse Bloom as Reviewing editor, as well as by two anonymous reviewers with substantial expertise in spindle positioning.
During mitosis, a select pool of MEK1 and p42/p44 MAPK becomes activated at the kinetochores and spindle poles, without substantial activation of the bulk of the cytoplasmic p42/p44 MAPK.
The substantial update incorporates the new Lexus corporate fascia, comprising the "spindle" grill and consequently sees the fitment of a redesigned hood, reshaped front fenders and headlamps, plus a new bumper.
Paul and Mary Klotman bought 387 West End in the 1990's, and have substantial portions of the original finishes: most of an intricate spindle-screen balustrade, swelling spiral colonettes on an oak mantelpiece, and a frieze of sunflowers on a wooden mantelpiece on the second floor.
When the spindle checkpoint is inactive, the loss of Ybp2 results in substantial chromosome loss, suggesting that the spindle checkpoint detects the mitotic defect and suppresses it by delaying mitosis.
Indeed, as shown by a laser cutting experiment by Tirnauer et al., the spindle has the ability to restore itself even after a substantial part of it has been removed [19].
However, there are substantial differences in the peak force that can be sustained before the spindle buckles.
When the spindle checkpoint is active, loss of Ybp2 does not cause a substantial mitotic defect but does cause a mitotic delay.
In contrast to the spindle shrinkage to a size of 6.3±2 µm observed upon TPX2 substantial depletion, large microtubule structures were observed upon co-injection with either YFP-TPX2 or YFP-TPX2ΔN (Fig 5B).
These findings may suggest that a substantial part of the CAV1 is transported from the HSC-3 cells into the spindle cells in the lower region.
