The histone and DNA methylation required to silence a newly integrated transposon or provirus occurs only after substantial silencing by deacetylation has already begun (Maksakova et al. 2008; Latham and Dent 2007; Mutskov and Felsenfeld 2004).
It is well known that genomes under stress, such as after a strong heat shock, undergo a substantial silencing, probably to avoid the potential danger of incorrect folding of proteins.
We found that mir-376a2 expressed and processed in the absence of cotransfected ADAR constructs generated substantial silencing activities corresponding to the mature, unedited 5′ and 3′ miRs, as expected, (Supplementary Figure S3B), reducing luciferase levels 10-fold to approximately 0.1 of the value obtained in the absence of the miRNA.
Second, while there is still more than one homoeologous copy for at least 84% of group 1 genes, only 69% of these genes are expressed from more than one copy in either shoot or root tissues, which could indicate substantial homoeolocus silencing.
Our previous work showed that the deletion of yKU70 causes a substantial reduction of silencing at both the truncated VIIL and the native XIL telomeres [ 17], and we have obtained similar results at XIL in a yku80Δ strain.
Deletion of the first GTGT repeat (vgΔ1st GT) at site 2 resulted in a substantial loss of silencing in both heterozygotes (twofold) and homozygotes (threefold), resulting in a twofold reduction in PSS.
Mr Zenawi clouded a gleaming reputation by conflating state and personal power, and used Ethiopia's fast-growing economy and substantial regional clout to silence his critics and get his way.
In one fell swoop, Obama could silence the substantial criticisms currently being lobbed against him.
This novel method of gene silencing has substantial promise for elucidation of gene function in bacterial species that have been refractory to experimental introduction of exogenous DNA.
This month, the leader of Cambodia – through a legal system his political party largely controls - abolished the only substantial opposition party and silenced much of the media that had become too critical.
This mutation leads to partial gene silencing and substantial reduction of the frataxin level.
