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Horse, bovine, and boar were chosen as these species are ungulates (like camel) with substantial sequence data and share many physiological properties with camel.
High-throughput SNP discovery, a touchstone of modern genomics, has traditionally relied on substantial sequence data and until recently has been considered impractical for uncharacterized species, especially those with large or complex genomes.
Therefore cross-species (heterologous) microarray studies using gDNA-based probe selection allows the profiling of up to ~58% of the total Musa genome despite the absence of substantial sequence data for this species and the large phylogenetic distance from the target species.
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RNA-Seq has thus been proven to act as a powerful approach for creating transcriptomic data for non-model plants, particularly those without substantial existing sequence data [ 19].
In the case of Tetrahymena, the lack of substantial comparative sequence data from near relatives and/or EST data led us to the use of ab initio methods that, while sufficient for a general analysis of genome contents [ 3], do not provide gene models of satisfactory quality for a widely studied model organism.
For example, proteome analysis of banana, which is distantly related to most plant species with substantial DNA sequence data, was used to compare various protein extraction methods and one-dimensional versus 2D SDS-PAGE techniques within the context of cross-species matching of peptide mass spectra.
The other two methods assigned a substantial amount of sequence data incorrectly to bins at the family level or below.
Though the substantial goat genome sequence data is currently short, it is feasible to identify putative miRNAs by means of alignment with goat expressed sequence tags (ESTs).
We sought to improve this situation using the substantial amount of sequence data now available from across eukaryotes, particularly from animals (Metazoa), green plants (Viridiplantae) and Fungi.
For non-model species lacking substantial genomic DNA sequence data, the set of conserved genes to be used for normalization can be selected according to high sequence conservation across more distantly related and sequenced organisms.
This effort has generated a substantial database of novel sequence data for these tissues as well as for S. salar itself.
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