To our knowledge, this is the first substantial sequencing and comparison of gut transcriptomes in whiteflies.
We sequenced a transcriptome of this ecologically and evolutionarily well-characterized species, returning substantial sequence diversity and expression information from a relatively shallow sequence coverage depth of long reads.
Horse, bovine, and boar were chosen as these species are ungulates (like camel) with substantial sequence data and share many physiological properties with camel.
For a protein like OPN that has substantial sequence redundancy and lack of secondary/tertiary structure, one solution is to divide the protein into virtual peptides, many of which have similar motifs, and analyze the interaction with a crystal face of each peptide separately.
Indeed, in Arabidopsis, duplication of the CDC27 subunit led to substantial sequence divergence and specialization [ 23].
In addition, the approximately 3.6 × 108 base pairs of data produced here represent a substantial sequence resource and will contribute to genomic data available for citrus.
The time since its birth was too short for substantial sequence diversification and the gene itself has not yet spread through the whole population.
High-throughput SNP discovery, a touchstone of modern genomics, has traditionally relied on substantial sequence data and until recently has been considered impractical for uncharacterized species, especially those with large or complex genomes.
If these were not tRNA genes, then it would seem extremely unlikely that the same relative stretch of DNA in both species would happen to have the potential to serve as the template for the same type of tRNA molecule, to exhibit substantial sequence similarity, and to have similar inferred secondary structures.
All the candidate gene sequences upon alignment with known databank sequences of MTBC, MAPC, MAIC and other non-tuberculous mycobacteria (NTMs), revealed substantial sequence similarities between MIP and MAC as well as MAIC bacilli.
